解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科，口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学，全科和内科
医学，法律
医学，研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学，核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学，临床
abstract：:The development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. The transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. It was also found to be an excellent enrichment medium for the selecti...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
abstract：:Eleven isolates of Aeromonas species from human stool cultures were found to produce enterotoxin activity as determined by assay of culture filtrates in rabbit intestinal loops and rabbit skin and on adrenal Y1 cells. Hemolysin(s) and a cytotoxic protein were found to interfere in all three assay systems but could be ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
abstract：:The synthetic anticoagulant sodium amylosulfate (SAS) at concentrations of 125 to 2,000 microgram/ml failed to completely neutralize the bactericidal activity of 80 and 50% (by volume) fresh human serum. Furthermore, SAS failed to inhibit the alternative pathway of complement activation in 80% (by volume) fresh human ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:The hexagonal surface structure believed to be characteristic for influenza C virus was not initially observed for a newly isolated virus of this type. After a few additional egg passages of the virus, however, the hexagonal structure could be readily seen on many virions. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the dis...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:A rapid and sensitive urease test for mycobacteria utilizing a cumulative radiometric technique is described. Definitive results were obtained within a 30-min incubation period. The procedure is simple and economical. Technical time involved is no greater than in conventional procedures. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:A recently described method for detecting rubella-specific immunoglobulin M (IgM) antibody based on absorption of IgG by Staphylococcus aureus strain Cowan I has been applied to 198 sera collected during a recent rubella epidemic in Israel. Modification of the original method introduced for the present study includes ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:With use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 Escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either E. coli or Vibrio cholerae. These findings suggest marked ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
abstract：:The Oxi-ferm tube, an eight-compartment system suitable for the recognition of nonfermentative, gram-negative, rod-shaped bacteria, was evaluated in conjunction with the oxidase test in a clinical microbiology laboratory setting. A comparison between this system and the 17-test routine examination was performed with 2...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:The frequency with which clue cells could be detected in Gram-stained vaginal smears and/or cervical Papanicolaou (Pap) smears was compared with the frequency of Corynebacterium vaginale (Haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. Vaginal clue cells were found most o...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The ac...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-03-01 00:00:00
abstract：:Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more ra...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:Treatment of wild rats from a known scrub typhus focus in the Philippines with cyclophosphamide did not significantly enhance the isolation rate of Rickettsia tsutsugamushi using mouse inoculation techniques. Similarly, cyclophosphamide treatment of mice inoculated with organs of wild rats did not increase the recover...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:Lysis and filtration of blood culture specimens were combined with impedance detection of bacterial growth to facilitate the diagnosis of bacteremia. A blood lysis-filtration technique (Zierdt et al., 1976) was coupled to a simple, inexpensive automated detection system. The practical and technical aspects of the impe...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-12-01 00:00:00
abstract：:One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Forty-five percent of 108 nontypable strains of Staphylococcus aureus from clinical and environmental sources were phage typable after heat shock and acridine orange treatment. Although phage patterns were produced by different types of treatment, the same phage groups were present, showing specificity. The newly typa...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:A study of immunofluorescence of yeast in the urine was carried out in 18 patients with funguria in an effort to correlate gamma globulin coating with evidence of tissue invasion or as an indicator for institution of therapy. Positive immunofluorescence of yeast in urine is common but is neither indicative of upper ur...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:Results of serotyping 966 clinical isolates of Pseudomonas aeruginosa showed that 72% agglutinated specifically in one or another of the 16 typing antisera, but 28% agglutinated in two or more and often in as many as 10 antisera; this polyagglutinability correlated with a high incidence of cross-reactivity among the a...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-08-01 00:00:00
abstract：:During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illne...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal gr...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not var...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-06-01 00:00:00
abstract：:The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing hu...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:The indirect immunoperoxidase antibody technique (IPA) has been applied to determine immunoglobulin (Ig)G to humans cytomegalovirus (CMV) antibodies in 114 blood donor sera, four cases of congenital cytomegalic inclusion disease, and four cases of acquired CMV infection. The results have been compared with those obtai...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:The epidemiology of hospital infections due to Pseudomonas aeruginosa was investigated by pyocin typing. The typing method, which determined the pyocin activity of clinical isolates of P. aeruginosa on 27 indicator strains, was 43.7% reproducible, but elimination of 9 indicator strains doubled the reproducibility and ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:Three antigenic variants of the H3N2 subtype of wild-type influenza A virus (representing the years 1968, 1972, and 1974) were examined for their sensitivity to interferon and for their ability to induce local respiratory tract interferon in volunteers. In addition, the time of appearance of symptoms in infected volun...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement betw...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-02-01 00:00:00
